&lt;h2&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;Overview&lt;/strong&gt;&lt;/span&gt;&lt;/h2&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Acetylcholinesterase (AChE) analysis in amniotic fluid is utilized to detect open neural tube defects and, to a lesser extent, ventral wall defects. The method involves Polyacrylamide Electrophoresis. It is available with the reporting name &quot;Acetylcholinesterase, AF&quot; or under aliases such as AChE-AF and Amniotic Fluid, Acetylcholinesterase.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;The specimen volume required is 1 mL, preferably collected during the 14- to 18-week gestational period, but acceptable up to 21 weeks. Additional testing requirements include referring to CHRAF for chromosome analysis and avoiding freezing the specimen when paired with chromosome analysis. Necessary information includes gestational age at amniocentesis and Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;The minimum specimen volume is 0.3 mL. Specimen stability varies depending on storage conditions.&lt;/span&gt;&lt;/p&gt;
&lt;hr /&gt;
&lt;h3&gt;&amp;nbsp;&lt;/h3&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;WHAT IS IT USED FOR?&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Identifying open neural tube defects, and to a lesser extent, ventral wall defects.&lt;/span&gt;&lt;/p&gt;

&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;METHOD NAME&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Polyacrylamide Electrophoresis&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;AVAILABILITY&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;TBC&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;REPORTING NAME&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;h4&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Acetylcholinesterase, AF&lt;/span&gt;&lt;/h4&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;ALIASES&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;AChE-AF (Acetylcholinesterase, Amniotic Fluid)&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Amniotic Fluid, Acetylcholinesterase&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;SPECIMEN TYPE&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Amniotic Fld&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;ADDITIONAL TESTING REQUIREMENTS&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;If chromosome studies are additionally needed, refer to CHRAF / Chromosome Analysis, Amniotic Fluid for details on specimen requirements. When paired with chromosome analysis, freezing the specimen is not possible.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;NECESSARY INFORMATION&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;ol&gt;
&lt;li&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt; Gestational age at amniocentesis is required.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #808080;&quot;&gt;&lt;span style=&quot;font-weight: 400;&quot;&gt;Second Trimester Maternal Screening Alpha-Fetoprotein / Quad Screen Patient Information&lt;/span&gt;&lt;span style=&quot;font-weight: 400;&quot;&gt; (T595) is required.&lt;/span&gt;&lt;/span&gt;&lt;/li&gt;
&lt;/ol&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;SPECIMEN REQUIRED&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Container/Tube: Amniotic fluid receptacle&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Specimen Volume: 1 mL&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Collection Instructions: Preferably collect the specimen during the 14- to 18-week gestational period. Amniotic fluid obtained between the 14- to 21-week gestational period is also acceptable.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;SPECIMEN MINIMUM VOLUME&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;0.3 mL&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;REJECT DUE TO&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;ul&gt;
&lt;li style=&quot;font-weight: 400;&quot; aria-level=&quot;1&quot;&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Gross hemolysis (OK)&lt;/span&gt;&lt;/li&gt;
&lt;li style=&quot;font-weight: 400;&quot; aria-level=&quot;1&quot;&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Gross icterus (OK)&lt;/span&gt;&lt;/li&gt;
&lt;/ul&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #33cccc;&quot;&gt;&lt;strong&gt;SPECIMEN STABILITY INFORMATION&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;ul&gt;
&lt;li style=&quot;font-weight: 400;&quot; aria-level=&quot;1&quot;&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Amniotic Fld &amp;ndash; Refrigerated (preferred) &amp;ndash; TBC days&lt;/span&gt;&lt;/li&gt;
&lt;li style=&quot;font-weight: 400;&quot; aria-level=&quot;1&quot;&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Amniotic Fld &amp;ndash; Frozen &amp;ndash; TBC days&lt;/span&gt;&lt;/li&gt;
&lt;li style=&quot;font-weight: 400;&quot; aria-level=&quot;1&quot;&gt;&lt;span style=&quot;font-weight: 400; color: #808080;&quot;&gt;Amniotic Fld &amp;ndash; Ambient &amp;ndash; TBC days&lt;/span&gt;&lt;/li&gt;
&lt;/ul&gt;

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