&lt;h2&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;OVERVIEW&lt;/strong&gt;&lt;/span&gt;&lt;/h2&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff; font-size: 14px;&quot;&gt;The &quot;Hemophilia A F8 Gene, Intron 1 and 22 Inversion Mutation Analysis, Prenatal&quot; test is conducted to detect specific mutations in the F8 gene associated with hemophilia A. This test is performed during pregnancy to determine if the fetus has inherited the genetic inversion mutations found in intron 1 and intron 22 of the F8 gene, which are common causes of hemophilia A. Detection of these mutations allows for early diagnosis and management of hemophilia A in the unborn child, providing valuable information for prenatal care and treatment planning.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;hr /&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;WHAT IS IT USED FOR?&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Testing for hemophilia A during pregnancy in families where no specific genetic mutation has been identified.&lt;/span&gt;&lt;/p&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;METHOD NAME&lt;/span&gt;&lt;br /&gt;&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Polymerase Chain Reaction (PCR) or Inverse Shifting-Polymerase Chain Reaction (IS-PCR)&lt;/span&gt;&lt;/p&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;AVAILABILITY&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;TBC&lt;/span&gt;&lt;/p&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;REPORTING NAME&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;div id=&quot;ReportingName-value&quot;&gt;
&lt;div id=&quot;ReportingName-value&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;HA F8 Int 1/22 Inversion, AF or CVS&lt;/span&gt;&lt;/div&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;OTHER NAME&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;ul&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Hemophilia A&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;HA&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Factor VIII gene&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;F8&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Intron 22 inversion&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Intron 1 inversion&lt;/span&gt;&lt;/li&gt;
&lt;/ul&gt;
&lt;/div&gt;

&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3 id=&quot;SpecimenType-title&quot;&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;SPECIMEN TYPE&lt;/strong&gt;&amp;nbsp;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Amniotic fluid&lt;/span&gt;&lt;/p&gt;
&lt;h2&gt;&amp;nbsp;&lt;/h2&gt;
&lt;h3&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;SHIPPING INSTRUCTIONS&lt;/span&gt;&lt;br /&gt;&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;TBC&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;h3&gt;&lt;span style=&quot;color: #ff99cc;&quot;&gt;&lt;strong&gt;SPECIMEN REQUIRED&lt;/strong&gt;&lt;/span&gt;&lt;/h3&gt;
&lt;p style=&quot;font-weight: 400;&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Container/Tube:&amp;nbsp;&lt;/strong&gt;Amniotic fluid container&amp;nbsp;&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Specimen Volume:&lt;/strong&gt; 5-10 mL&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Collection Instructions:&lt;/strong&gt;&lt;/span&gt;&lt;/p&gt;
&lt;ol&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;The best time to collect the specimen is between 14 to 18 weeks of pregnancy, but samples from other weeks are also acceptable.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Remove the initial 2 mL of amniotic fluid. If chromosome analysis and alpha-fetoprotein tests are included, a total of about 25 mL to 30 mL is required for both studies.&lt;/span&gt;&lt;/li&gt;
&lt;/ol&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Additional Information:&lt;/strong&gt;&lt;/span&gt;&lt;/p&gt;
&lt;ol&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Place tubes in a Styrofoam container.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Fill any remaining space with packing material.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Approximately 1% to 2% of mailed-in specimens are not viable.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Avoid sending bloody specimens.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;If the specimen doesn&#39;t culture, notification will occur within 7 days of receipt.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Additional culture fees apply under CULAF / Culture for Genetic Testing, Amniotic Fluid.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;All prenatal samples must include a maternal blood specimen; request MATCC / Maternal Cell Contamination, Molecular Analysis, Varies for maternal testing.&lt;/span&gt;&lt;/li&gt;
&lt;/ol&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;p style=&quot;font-weight: 400;&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Specimen Type:&amp;nbsp;&lt;/strong&gt;Chorionic villi&amp;nbsp;&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;font-weight: 400;&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Container/Tube:&lt;/strong&gt; 15-mL tube containing 15 mL of transport media&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Specimen Volume:&lt;/strong&gt; 20-30 mg&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Collection Instructions:&lt;/strong&gt;&lt;/span&gt;&lt;/p&gt;
&lt;ol&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Gather the sample through transabdominal or transcervical means.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Place the chorionic villi into a Petri dish with transport medium.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Inspect the villi&#39;s quality and quantity under a stereomicroscope, removing any blood clots and maternal tissue with sterile forceps.&lt;/span&gt;&lt;/li&gt;
&lt;/ol&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Additional Information:&lt;/strong&gt;&lt;/span&gt;&lt;/p&gt;
&lt;ol&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;An additional culture fee applies under CULFB / Fibroblast Culture for Biochemical or Molecular Testing. Culturing fibroblasts adds 3 to 4 weeks before genetic testing.&lt;/span&gt;&lt;/li&gt;
&lt;li&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;Every prenatal sample must include maternal blood; request MATCC / Maternal Cell Contamination, Molecular Analysis, Varies for maternal testing.&lt;/span&gt;&lt;/li&gt;
&lt;/ol&gt;
&lt;p&gt;&amp;nbsp;&lt;/p&gt;
&lt;p style=&quot;font-weight: 400;&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Specimen Type:&amp;nbsp;&lt;/strong&gt;Confluent cultured cells&amp;nbsp;&lt;/span&gt;&lt;/p&gt;
&lt;p style=&quot;font-weight: 400;&quot;&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Container/Tube:&amp;nbsp;&lt;/strong&gt;T-25 flask&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Specimen Volume:&lt;/strong&gt; 2 Flasks approximately 90% confluent&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Collection Instructions:&amp;nbsp;&lt;/strong&gt;Provide fully grown cultured cells from an external laboratory.&lt;/span&gt;&lt;/p&gt;
&lt;p&gt;&lt;span style=&quot;color: #99ccff;&quot;&gt;&lt;strong&gt;Additional Information:&amp;nbsp;&lt;/strong&gt;Every prenatal sample requires a maternal blood specimen; request MATCC / Maternal Cell Contamination, Molecular Analysis, as applicable to the maternal sample.&lt;/span&gt;&lt;/p&gt;